Resources

Protocols

- Husbandry

• Collection and storage of Nfur embryos
• Hatching Nfur embryos
• Preparing brine shrimps
• Shipping fish
• Killfish room guideline

- Experiments

• Cell dissociation from Nfur embryos
• ChIP-seq with Nfur embryos
• Comet assay of cells from Nfur embryos
• Designing peptides for peptide antibodies
• DNA extraction from Nfur embryos
• DNA extraction from Nfur tail tissues
• Genotyping Nfur embryos or caudal fin tissues
• gRNA synthesis and purification
• Immunostaining Nfur embryos
• in situ HCR v3.0 with Nfur embryos
• Passive CLARITY for Nfur brains
• Preparation of methylcellulose mounting solution
• Protein extraction from Nfur embryos
• RNA extraction from Nfur embryos

Prints

(We are happy to customize our prints or design new tools for other systems and research. The only condition is to agree sharing with the community. If interested, contact us!)

- Dish holder

• Base: (2mm) & (4mm) / Arm / Arm with label: (5cm) & (10cm) / Connector / Arm ring - Info & Photos

- Embryo shipping plate

• Grid / Cap / Band1 / Band2 - Info & Photos & Assembly intro

- Feeding funnel

• Aquaneering_A for 1.4 & 2.8L tanks / Aquaneering_B for 1.8L tanks / Aquaneering C for 0.8L tanks- Info & Photos

- Feeder (need to couple with the feeding funnel)

• 35mg Feeder: (left-handed) & (right-handed) / 20mg Feeder: (left-handed) & (right-handed) / Feeder adapter / Feeder cap - Info & Photos

- Freezer doorlock

• Bar / Base / Stopper - Info & Photos

- Fridge/freezer label

• Label / Label arrow: (Up), (Down), (Left) & (Right) / Label holding lock - Info & Photos

- Gel extractor

• Gel extractor - Info & Photos

- Gel holder and shovel

• Gel holder: (long mini gels) & (short mini gels) / Gel shovel - Info & Photos

- Gliding table

• Base plate / Bottom gear / Extension table / Gear axel / Gear knob / Middle bar / Top gear / Top plate / Wahser - Info & Photos & Assembly_intro

- Holder and hanger

• Fixed holder: (19mm), (19.5mm), (24mm), (70mm), (71mm) / Adjustable holder, Skrew (25mm), (40mm) / Attachable holder, Cap / Tube adaptor / Fixed hanger: (70mm), (71mm) - Info & Photos

- Injection molds

• Regular / minimal support - Info & Photos

- Micro spoon sets

• Spoon(L)-Splash / Spoon(M)-Dribble / Spoon(S)-Bit - Info & Photos

- Pipette tip ejector

• Pipette tip ejector for Gilson p2, p10, and p20 - Info & Photos

- Tube opener

• Tube opener - Info & Photos

- Tweezer limiter

• Fixed / Adjustable - Info & Photos