Protocols
- Husbandry
- Collection and storage of Nfur embryos
- Hatching Nfur embryos
- Preparing brine shrimps
- Shipping fish
- Killfish room guideline
- Experiments
- Cell dissociation from Nfur embryos
- ChIP-seq with Nfur embryos
- Comet assay of cells from Nfur embryos
- Designing peptides for peptide antibodies
- DNA extraction from Nfur embryos
- DNA extraction from Nfur tail tissues
- Genotyping Nfur embryos or caudal fin tissues
- gRNA synthesis and purification
- Immunostaining Nfur embryos
- Preparation of methylcellulose mounting solution
- Protein extraction from Nfur embryos
- RNA extraction from Nfur embryos
Prints
(We are happy to customize our prints or design new tools for other systems and research. The only condition is to agree sharing with the community. If interested, contact us!)
- Injection molds
- Regular / minimal support - Info & Photos
- Feeding funnels
- Aquaneering_A for 1.4 & 2.8L tanks / Aquaneering_B for 1.8L tanks / Aquaneering C for 0.8L tanks- Info & Photos
- Feeders (need to couple with the feeding funnels)
- 35mg Feeder_left-handed / 35mg Feeder_right-handed / 20mg Feeder_left-handed / 20mg Feeder_right-handed / Feeder adapter / Feeder cap - Info & Photos
- Embryo shipping plate
- Embryo shipping grid / Embryo shipping cap / Embryo shipping band1 / Embryo shipping band2 - Info & Photos & Assembly intro
- Freezer doorlock
- Gliding table
- Base plate / Bottom gear / Extension table / Gear axel / Gear knob / Middle bar / Top gear / Top plate / Wahser - Info & Photos & Assembly_intro