1) Killifish lines
We are providing following African killifish lines. Please let us know if interested in specific lines.
- DBL-D
- Inbred line (>25 sibling crossing, from the GRZ line, yellow tail morph)
- Originally captured in Zimbabwe (-21° 40' 36.00", +31° 44' 0.00"), 1970
- The reference line for both Stanford and Jena killifish genome assemblies
- DBL-W
- Inbred line (>25 sibling crossing, from the ZMZ1001 line, yellow tail morph)
- Originally captured in Mozambique/Zimbabwe border (-21° 48' 55.98", +31° 55' 52.32"), 2010
- Captured spot 25 km away from the GRZ spot
- DBL-J
- Inbred line (>25 sibling crossing, from the ZMZ1002 line, yellow tail morph)
- Originally captured in Mozambique (-21° 55' 0.66", +32° 5' 52.14"), 2010
- Captured spot 48 km away from the GRZ spot
- DBL-M
- Inbred line (>25 sibling crossing, from the MZM0403 line, red tail morph)
- Originally captured in Mozambique (-23° 0' 0.00", +32° 0' 0.00"), 2004
2) Protocols
- Husbandry
- Collection and storage of Nfur embryos
- Hatching Nfur embryos
- Preparing brine shrimps
- Shipping fish
- Killfish room guideline
- Experiments
- Cell dissociation from Nfur embryos
- ChIP-seq with Nfur embryos
- Comet assay of cells from Nfur embryos
- Designing peptides for peptide antibodies
- DNA extraction from Nfur embryos
- DNA extraction from Nfur tail tissues
- Genotyping Nfur embryos or caudal fin tissues
- gRNA synthesis and purification
- Immunostaining Nfur embryos
- in situ HCR v3.0 with Nfur embryos
- Passive CLARITY for Nfur brains
- Preparation of methylcellulose mounting solution
- Protein extraction from Nfur embryos
- RNA extraction from Nfur embryos
3) Prints
(We are happy to customize our prints or design new tools for other systems and research. The only condition is to agree sharing with the community. If interested, contact us!)
All .stl files are using the scale of “mm” instead of “cm”. Thus might need to scale up 10x when slicing if your slicer default is “cm”.
- Agarose scoop
- Scoop size (0.5g) / (0.6g) / (1g) - 0.6g Inventor file, Info & Photos
- Dish holder
- Base: (2mm) & (4mm) / Arm / Arm with label: (5cm) & (10cm) / Connector / Arm ring - Inventor files, Info & Photos
- Dovetailed tube rack
- Tube rack: (0.2ml) / (0.6ml) / (1.5ml & 2ml) / (5ml & 15ml) & (50ml) - Inventor files, Info & Photos
- Embryo shipping plate
- Feeding funnel
- Aquaneering_A for 1.4 & 2.8L tanks / Aquaneering_B for 1.8L tanks / Aquaneering C for 0.8L tanks- Info & Photos
- Feeder (need to couple with the feeding funnel)
- 35mg Feeder: (left-handed) & (right-handed) / 20mg Feeder: (left-handed) & (right-handed) / Feeder adapter / Feeder cap - Info & Photos
- Freezer doorlock
- Fridge/freezer label
- Label / Label arrow: (Up), (Down), (Left) & (Right) / Label holding lock - Info & Photos
- Fry measuring plate
- Gel extractor
- Gel extractor - Info & Photos
- Gel holder and shovel
- Gliding table
- Base plate / Bottom gear / Extension table / Gear axel / Gear knob / Middle bar / Top gear / Top plate / Wahser - Info, Photos & Assembly_intro
- Holder and hanger
- Fixed holder: (19mm), (19.5mm), (24mm), (70mm), (71mm) / Adjustable holder, Skrew (25mm), (40mm) / Attachable holder, Cap / Tube adaptor / Fixed hanger: (70mm), (71mm) - Info & Photos
- Injection molds
- Micro spoon sets
- Pipette tip ejector
- Tape holder holder
- Tube opener
- Tube opener - Info & Photos
- Tweezer limiter
- Fixed / Adjustable - Info & Photos